mAb anti-hGH, YC6, Capture

Description

Description: Mouse monoclonal antibody to human growth hormone (hGH)

Purification: Protein G affinity purified

Product Type: Primary antibody – capture antibody in matched antibody pair

Target Protein: Human growth hormone (hGH)

Immunogen: Purified recombinant hGH

Fusion Myeloma: Sp2/0-Ag14

Specificity: This monoclonal antibody reacts with natural and recombinant hGH.  The affinity constant measured by Friguet’s method (1985) is 1.7 x 10¹⁰/M.

Species Reacitvity: Human, others not tested

Host / Isotype: Mouse, IgG2a Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.2

Reconstitution: Double distillated water is recommended to adjust the final concentration to 1.00 mg/mL.

Storage: Store at -20^oC

Research Area: Hormone, growth and development

Background: 

Human growth hormone (hGH) is a 191 amino acid polypeptide secreted by the pituitary gland in the brain.  The hormone is critical for height increase and stature development before puberty, and is needed for maintaining healthy organ and vitality in adults.  hGH inadequacy during childhood results in short stature and over secretion of hGH causes gigantism.  hGH is regulated primarily by somatocrinin and somatostatin, which are released from hypothyalamus in response to physiological signals such as age, gender nutrition, excise, stress and sleep etc.

Application: 

ELISA: This antibody can be used as a capture antibody in sandwich ELISA applications for hGH detection in combination with anti-hGH monoclonal antibody clone YC8 (HRP-conjugated, Cat. No.: MO-M40012T or Biotin-conjugated, Cat. No.: MO-M40012TB).

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.