mAb anti-Human α1-antitrypsin, 5G5

Description

Description: Mouse monoclonal antibody to human α1-antitrypsin (AAT)

Purification: Protein G affinity purified

Product Type: Primary antibody

Target Protein: Human α1-antitrypsin

Immunogen: Human plasma derived α1-antitrypsin

Fusion Myeloma: Sp2/0-Ag14

Specificity: This monoclonal antibody reacts with human α1-antitrypsin.

Species Reacitvity: Human

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.2

Reconstitution: Double distillated water is recommended to adjust the final concentration to 1.00mg/mL. 

Storage: Store at -20^oC

Research Area: α1-antitrypsin deficiency disorder, Alzheimer’s disease

Background:  

α1-antitrypsin (AAT) is a protective protease inhibitor.  It inhibits elastase released by neutrolphils during inflammatory reaction and irreversibly inactivates trypsin secreted in duodenum and elsewhere.  α1-antitrypsin normally presents in plasma at 1.5-3.5 mg/mL, but increases in acute phase reaction, malignancies, pregnancy and trauma.    Severe hereditary a1-antitrypsin deficiency can result in damages to alveoli, chronic obstructive pulmonary disease (COPD), and liver diseases such as jaundice and cirrhosis.  The protein is also found in the neurofibrillary tangles and senile plaques in the brain lesions of Alzheimer’s disease, and has been suggested to contribute to the pathology of Alzheimer’s disease.

Application: 

1. Western Blot: Detect α1-antitrypsin (AAT) using antibody 5G5 at 1:5000 dilution (0.2 μg/mL).

2. ELISA 

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.