mAb anti-Human IL-10, 4H2

Description

Description: Mouse monoclonal antibody to human interleukin-10 (IL-10)

Purification: Protein G affinity purified

Target Protein: Human IL-10

Immunogen: Purified recombinant human IL-10 (Insight genomics)

Fusion Myeloma: F0

Specificity: Reactive with human IL-10.  

Species Reactivity: Human, others not tested.

Host / Isotype: Mouse, IgG2b Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.0

Reconstitution: Double distilled water is recommended to reconstitute the antibody.

Storage: Store at -20oC

Research Area: Cytokine, growth factor, inflammatory and immune responses

Background:

IL-10 is expressed by a variety of cells and plays an important role in inflammatory and immune responses. The biological activities of IL-10 include both immunosuppressive and immuno-stimulatory effects.  It suppresses the production of pro-inflammatory cytokines by monocytes and neutrophils, and down-regulates the expression of activating and co-stimulatory molecules on monocytes and dendritic cells.  It stimulates the growth of B cells and mast cells, and inhibits or enhances the activities of T cells depending on their activation conditions.  Human IL-10 is a protein with 160 amino acids. 

Applications: 

Indirect ELISA:  The anti-human IL-10 antibody clone 4H2 reacts with recombinant human IL-10. 

Western blot:  The anti-human IL-10 antibody clone 4H2 reacts with recombinant human IL-10 antigen (Immunogen) (Figure 1) and with human IL-10 expressed in 293T cells (Figure 2).

Lane 1&2:  Recombinant human protein loaded at 10ng/well.   Anti-human IL-10 clone 4H2 was used at 1:5,000 dilution for Western blot.

Lane 1:  Un-transfected 293T cells; lane 2: 239T cells transfected with human IL-6 gene; lane 3: 293T cells transfected with human IL-10 gene.   Anti-human IL-10 antibody 4H2 was used at 1:2,000 dilution for Western blot.

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.