mAb anti-Human IL-6, 1B6

Description

Description: Mouse monoclonal antibody to human interleukin-6 (IL-6)

Purification: Protein G affinity purified

Target Protein: Human IL-6

Immunogen: Purified recombinant human IL-6

Fusion Myeloma: F0

Specificity: Reactive with natural and recombinant human IL-6.  

Species Reactivity: Human, others not tested.

Host / Isotype: Mouse, IgG1 Kappa

Formulation: Lyophilized from a solution in 0.01M PBS, pH 7.0

Reconstitution: Double distillated water is recommended to adjust final concentration to 1 mg/mL.

Storage: Store at -20oC

Research Area: Cytokine, growth factor, inflammatory and autoimmune diseases

Background:

IL-6, also called B-cell stimulatory factor-2 (BSF-2), is an important cytokine for the differentiation of B-cells into immunoglobulin-secreting cells.  IL-6 is mainly produced by T-cells, macrophages and endothelial cells in response to pathogenic molecular stimuli during infection, trauma and the growth phase of some types of cancer. IL-6 is up-regulated by the binding of Toll-like receptors to antigen pattern ligands.  In acute inflammatory phase, IL-6 causes increased body temperature by initiating Prostaglandin E2 synthesis in hypothalamus. 

Applications: 

ELISA: The antibody reacts with recombinant IL-6 antigen.

Western blot:  The antibody when used at 200ng/mL, can recognize 25ng/lane recombinant human IL-6 (rhIL-6) on blot.  Lane 1 and 2: rhIL-6 loaded at 25ng/lane, Lane 3 and 4: rhIL-6 loaded at 50ng/lane. (See image)

Fluorescence flow cytometry: Above is the histogram of IL-6 transfected 293T cells stained with IL-6 mAb 1B6 (10μg/mL) and fluorescence-labelled secondary antibody.  Black line represents the histogram of IL-6 transfected 293T stained with isotype control, mouse anti-TB 38Kd antigen mAb clone B12F8 (Cat. No. MO-I40021D).

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.